General Information

Vol. per Test: 2 µL

Reactivity: Mouse(C57BL/6;BALB/c)

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Application: Flow cytometry

Stability & Storage:

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. 

Product Notices

1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use

1 × 10^6 cells in a 100-μL experimental sample

(a test).

2. Isotype control is not needed because the antibody has no Fc region.

3. For Research Use Only.

QC Testing:

Flow cytometric analysis of CD19 expression on mouse peripheral blood lymphocytes.

Peripheral blood mononuclear cell (PBMC) was stained with Anti-mouse CD19  Recombinant Antibody （GFP tag）. Erythrocytes were lysed with Lysing Buffer. The two-parameter dot plot showing CD19 expression were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.

Flow cytometric analysis of CD19 expression on mouse peripheral blood leucocyte populations

Mouse whole blood was stained with either Control Antibody or Anti-mouse CD19  Recombinant Antibody （GFP tag）. Erythrocytes were lysed with Lysing Buffer. Bivariate pseudocolor density plots showing the correlated expression of CD19 [or control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations.

Description

The antibody reacts with CD19, a B lymphocyte-lineage differentiation antigen. CD19, a 95-kDa transmembrance glycoprotein, is amember of the immunoglobulin superfamily and is expressed throughout B-lymphocyte development from the pro-B cell through the matureB-cell stages. Terminally differentiated plasma cells do not express CD19. On the surface of mature B cells, the CD19 molecule associateswith CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to promote cellularactivation. Studies with CD19-deficient mice have suggested that the level of CD19 expression affects the generation and maturation of B cellsin the bone marrow and periphery

References

1、Schlossman SF, Boumsell L, Gilks W, et al, ed. Leukocyte Typing V: White Cell Differentiation Antigens. New York: Oxford University Press; 1995.

 2、Knapp W, Dorken B, Rieber EP, et al, ed. Leucocyte Typing IV. New York: Oxford University Press; 1989.