General Information

Vol. per Test: 2 µL

Reactivity: Mouse(C57BL/6;BALB/c)

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Application: Flow cytometry

Stability & Storage:

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. 

Product Notices

1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use

1 × 10^6 cells in a 100-μL experimental sample

(a test).

2. Isotype control is not needed because the antibody has no Fc region.

3. For Research Use Only.

QC Testing:

Flow cytometric analysis of CD8 expression on mouse peripheral blood lymphocytes.

Peripheral blood mononuclear cell (PBMC) was stained with Anti-mouse CD8  Recombinant Antibody （GFP tag）. Erythrocytes were lysed with Lysing Buffer. The two-parameter dot plot showing CD8 expression were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.

Flow cytometric analysis of CD8 expression on mouse peripheral blood leucocyte populations

Mouse whole blood was stained with either Control Antibody or Anti-mouse CD8  Recombinant Antibody （GFP tag）. Erythrocytes were lysed with Lysing Buffer. Bivariate pseudocolor density plots showing the correlated expression of CD8 [or control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations.

Description

The monoclonal antibody specifically binds to the CD8 differentiation antigen of all mouse strains tested. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer. Subsets of γδ TCR-bearing T cells, intestinal intrapithelial lymphocytes, and dendriticcells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocyteswhich matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals. CD8 isan antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells.

References

1、Schlossman SF, Boumsell L, Gilks W, et al, ed. Leukocyte Typing V: White Cell Differentiation Antigens. New York: Oxford University Press; 1995.

 2、Knapp W, Dorken B, Rieber EP, et al, ed. Leucocyte Typing IV. New York: Oxford University Press; 1989.