General Information

Vol. per Test: 2 µL

Alternate Name: PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5

Reactivity: Human

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Application: Flow cytometry

Stability & Storage:

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. 

Product Notices

1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use

1 × 10^6 cells in a 100-μL experimental sample

(a test).

2. Isotype control is not needed because the antibody has no Fc region.

3. For Research Use Only.

QC Testing:

Flow cytometric analysis of CD45 expression on human peripheral blood lymphocytes.

Peripheral blood mononuclear cell (PBMC) was stained with Anti-human CD45  Recombinant Antibody （YFP tag）. Erythrocytes were lysed with Lysing Buffer. The two-parameter dot plot showing CD45 expression were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.

Flow cytometric analysis of CD45 expression on human peripheral blood leucocyte populations

Human whole blood was stained with either Control Antibody or Anti-human CD45  Recombinant Antibody （YFP tag）. Erythrocytes were lysed with Lysing Buffer. Bivariate pseudocolor density plots showing the correlated expression of CD45 [or control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations.

Description

The monoclonal antibody specifically binds to the 180, 190, 205, 220 kDa protein isoforms of CD45. CD45 is encoded by the PTPRC (Protein tyrosine phosphatase receptor type C) gene. CD45, also known as the leukocyte common antigen (LCA), is a member of the protein tyrosine phosphatase (PTP) family. It is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes. CD45 is absent from circulating erythrocytes, platelets, or mature erythroid cells of bone marrow and non-hemopoietic tissues.

References
1、Wang Y, Liang L, Esselman WJ. Regulation of the calcium/NF-AT T cell activation pathway by the D2 domain of CD45. J Immunol. 2000; 164(5):2557-2564. (Biology)
2、Wu L, Fu J, Shen SH. SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription. Mol Biol Cell. 2002; 22(8):2673-2686. (Biology)