General Information

Vol. per Test: 2 µL

Alternate Name:CSF2RA;GM-CSF Receptor alpha;GM-CSFRα;GMCSFRA;GMR, SMDP4

Reactivity: Human

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Application: Flow cytometry

Stability & Storage:

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use  1 × 10^6 cells in a 100-μL experimental sample  (a test).

2. Isotype control is not needed because the antibody has no Fc region.

3. For Research Use Only.

QC Testing:

Flow cytometric analysis of CD116 expression on human peripheral blood lymphocytes.

Peripheral blood mononuclear cell (PBMC) was stained with Anti-human CD116  Recombinant Antibody （GFP tag）. Erythrocytes were lysed with Lysing Buffer. The two-parameter dot plot showing CD116 expression were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.

Flow cytometric analysis of CD116 expression on human peripheral blood leucocyte populations

Human whole blood was stained with either Control Antibody or Anti-human CD116  Recombinant Antibody （GFP tag）. Erythrocytes were lysed with Lysing Buffer. Bivariate pseudocolor density plots showing the correlated expression of CD116 [or control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations.

Description

CD116 is expressed by a variety of myeloid cell lines,hematopoietic and non-hematopoetic tumor cells, and normal cell types including monocytes, macrophages, neutrophils,eosinophils, myeloid dendritic cells, endothelial cells, fibroblasts, and placental trophoblasts.  Lymphocytes are negativefor GM-CSFR expression.  

References

1、Jokhi PP, King A, Jubinsky PT, Loke YW.  Demonstration of the low affinity alpha subunit of the granulocyte-macrophage colony-stimulating factor receptor (GM-CSF-R alpha) on human trophoblast and uterine cells.  J Reprod Immunol. 1994;
2、Lanza F, Moretti S, Papa S, Malavasi F, Castoldi G. Report on the Fifth International Workshop on Human Leukocyte

Differentiation Antigens, Boston, November 3-7, 1993..  Haematologica. 79(4):374-86.  (Clone-specific: Flow cytometry).