General Information

Vol. per Test: 2 µL

Alternate Name:SDC1; Syndecan 1; Syndecan-1

Reactivity: Human

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Application: Flow cytometry

Stability & Storage:

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use  1 × 10^6 cells in a 100-μL experimental sample  (a test).

2. Isotype control is not needed because the antibody has no Fc region.

3. For Research Use Only.

QC Testing:

Flow cytometric analysis of CD138 expression on human peripheral blood lymphocytes.

Peripheral blood mononuclear cell (PBMC) was stained with Anti-human CD138 Recombinant Antibody （GFP tag）. Erythrocytes were lysed with Lysing Buffer. The two-parameter dot plot showing CD138 expression were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. 

Flow cytometric analysis of CD138 expression on human peripheral blood leucocyte populations

Human whole blood was stained with either Control Antibody or Anti-human CD138 Recombinant Antibody （GFP tag）. Erythrocytes were lysed with Lysing Buffer. Bivariate pseudocolor density plots showing the correlated expression of CD138 [or control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations.

Description

The MI15 monoclonal antibody specifically binds to CD138 (Syndecan-1), an 85-92 kDa single chain transmembrane protein, stronglyexpressed on multiple-myeloma-derived cell lines and malignant plasma cell populations. CD138 binds to many extracellular matrix proteins through its heparan sulfate side-chains, like fibronectin,collagen types I, III, and V, tenascin, thrombospondin, and antithrombin III.

References

1、Gattei V, Godeas C, Degan M, Rossi FM, Aldinucci D, Pinto A. Characterization of anti-CD138 monoclonal antibodies as tools for investigating the molecular

polymorphism of syndecan-1 in human lymphoma cells. Br J Haematol. 1999; 104(1):152-162. (Clone-specific: Flow cytometry, Immunoprecipitation)

2、Wijdenes J, Clément C, Klein B, Dore J-M. CD138 (syndecan-1) Workshop Panel report. In: Kishimoto T, Kikutani H, vond dem Borne AEGK, ed. LeukocyteTyping VI: White Cell Differentiation Antigens. New York: Garland Publishing Inc; 1998:249-252. (Clone-specific: Flow cytometry)